H3F3B AntibodyRabbit Polyclonal
|In-house applications||ELISA, WB Application not tested? Please see our Guarantee.|
|Published applications||Publications||0 citations –view here|
|Species specificity||Human, Mouse, Rat; other species not tested.|
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells|
|Recommended dilution||WB : 1:200-1:1000|
IP = Immunoprecipitation
|Source||Rabbit||Purification method||Antigen affinity purification|
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Aliquoting is unnecessary for -20oC storage
|Immunogen||H3F3B fusion protein ag0326||Full name||H3 histone, family 3B (H3.3B)|
|BC001124||Gene ID (NCBI)||3021|
|Gene symbol||H3F3B||Synonyms||H3 histone, family 3B (H3.3B), H3.3A, H3.3B, H3F3, H3F3A, H3F3B, Histone H3.3|
Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. H3F3B is one replacement histone [PMID: 8586426]. It constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Thereby it has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability [PMID: 14718166, 15776021].