Histone H3.3 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 13754-1-AP

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Con: 49 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
A431 cells, HEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse thymus tissue

Positive IF detected in:
A431 cells

Recommended dilution:
WB : 1:500-1:2000
IF : 1:50-1:500

Product Information


Purification method:
Antigen affinity purification


PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Full name:
H3 histone, family 3A

Calculated molecular weight:
136aa,15 kDa

Observed molecular weight:
15-17 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol

H3 histone, family 3A, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, Histone H3.3

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].

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