Histone H3.3 AntibodyRabbit Polyclonal
|In-house applications||ELISA, WB, IF Application not tested? Please see our Guarantee.|
|Published applications||Publications||0 citations –view here|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Positive WB detected in||A431 cells, HEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse thymus tissue|
|Positive IF detected in||A431 cells|
|Recommended dilution||WB : 1:500-1:5000|
IF : 1:50-1:500
IP = Immunoprecipitation
|Source||Rabbit||Purification method||Antigen affinity purification|
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Aliquoting is unnecessary for -20oC storage
|Immunogen||Histone H3.3 fusion protein ag4696||Full name||H3 histone, family 3A|
|BC038989||Gene ID (NCBI)||3020|
|Gene symbol||H3F3A||Synonyms||H3 histone, family 3A, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, Histone H3.3|
Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].