H2AFX Antibody
Rabbit Polyclonal
Catalog number: 10856-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | Raji cells, HEK-293 cells, HL-60 cells, mouse heart tissue |
| Positive IHC detected in | human lymphoma |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Raji cells were subjected to SDS PAGE followed by western blot with 10856-1-AP(H2AFX Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human lymphoma using 10856-1-AP(H2AFX Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using H2AFX antibody 10856-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | H2AFX fusion protein ag1305 | Full name | H2A histone family, member X |
|
Calculated molecular weight |
15kd |
Observed molecular
weight |
15-18kd |
|
GenBank accession number |
BC013416 | Gene ID (NCBI) | 3014 |
| Gene symbol | H2AFX | Synonyms | H2A.X; H2A/X; H2AX |
Background
Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. |
