Histone H2A.X AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, FC|
|Cited applications||IF, WB|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Cited species||human, rat|
|Positive WB detected in||Raji cells, HEK-293 cells, HL-60 cells, mouse heart tissue|
|Positive IHC detected in||human lymphoma tissue|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- Raji cells were subjected to SDS PAGE followed by western blot with 10856-1-AP(H2AFX antibody) at dilution of 1:1000
- Immunohistochemical of paraffin-embedded human lymphoma using 10856-1-AP(H2AFX antibody) at dilution of 1:100 (under 10x lens)
- Immunofluorescent analysis of HepG2 cells, using H2AFX antibody 10856-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- 1X10^6 HepG2 cells were stained with 0.2ug Histone H2A.X antibody (10856-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||Histone H2A.X fusion protein ag1305||Full name||H2A histone family, member X|
|BC013416||Gene ID (NCBI)||3014|
|Gene symbol||H2AFX||Synonyms||H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x|
Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.