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Galectin 10 Polyclonal antibody

Galectin 10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25225-1-AP

Synonyms

Charcot Leyden crystal protein, CLC, Eosinophil lysophospholipase, Gal 10, Galectin 10, LGALS10, LPPL_HUMAN, Lysolecithin acylhydrolase

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using Galectin 10 Polyclonal antibody (25225-1-AP)

    WB analysis of HL-60 using 25225-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25225-1-AP (Galectin 10 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using Galectin 10 Polyclonal antibody (25225-1-AP)

    IHC staining of human lung using 25225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using Galectin 10 Polyclonal antibody (25225-1-AP)

    IHC staining of human lung using 25225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Galectin 10 Polyclonal antibody (25225-1-AP)

    IHC staining of human spleen using 25225-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Galectin 10 Polyclonal antibody (25225-1-AP)

    IHC staining of human spleen using 25225-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Galectin 10 Polyclonal antibody (25225-1-AP)

    IF Staining of THP-1 using 25225-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Galectin 10 antibody (25225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25225-1-AP is Galectin 10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin 10 fusion protein Ag18569
Full Name Charcot-Leyden crystal protein
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC119711
Gene Symbol Galectin 10
Gene ID (NCBI) 1178
RRIDAB_2879973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Galectin 10 antibody 25225-1-APDownload protocol
IHC protocol for Galectin 10 antibody 25225-1-APDownload protocol
IF protocol for Galectin 10 antibody 25225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Syst Biol Reprod Med

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Authors - Genhai Zhu
View Article
humanIHC

Int J Surg Pathol

Langerhans Cell Histiocytosis With Unusual Hexagonal Crystals in Addition to Usual Charcot-Leyden Crystals. Report of a Patient With Possible Process of Crystal Formation and Clinical Significance of a "Necrotic" Change

Authors - Sayaka Ando
View Article
humanIF

Cells

CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.

Authors - Ssu-Ju Fu
View Article