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TFII I Polyclonal antibody

TFII I Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10499-1-AP

Synonyms

BTKAP1, BTK associated protein 135, Bruton tyrosine kinase-associated protein 135, BAP-135, BAP135

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFII I Polyclonal antibody (10499-1-AP)

    WB analysis using 10499-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TFII I Polyclonal antibody (10499-1-AP)

    WB analysis of HeLa using 10499-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TFII I Polyclonal antibody (10499-1-AP)

    WB analysis of Jurkat using 10499-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TFII I Polyclonal antibody (10499-1-AP)

    WB analysis of HEK-293 using 10499-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TFII I Polyclonal antibody (10499-1-AP)

    IHC staining of human thyroid cancer using 10499-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TFII I Polyclonal antibody (10499-1-AP)

    IHC staining of human thyroid cancer using 10499-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using TFII I Polyclonal antibody (10499-1-AP)

    IHC staining of human urothelial carcinoma using 10499-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using TFII I Polyclonal antibody (10499-1-AP)

    IHC staining of mouse small intestine using 10499-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using TFII I Polyclonal antibody (10499-1-AP)

    IHC staining of rat kidney using 10499-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using TFII I Polyclonal antibody (10499-1-AP)

    IHC staining of rat small intestine using 10499-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TFII I Polyclonal antibody (10499-1-AP)

    IHC staining of human colon cancer using 10499-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFII I Polyclonal antibody (10499-1-AP)

    IF Staining of HeLa using 10499-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10499-1-AP (TFII I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFII I Polyclonal antibody (10499-1-AP)

    IF Staining of HeLa using 10499-1-AP

    Immunofluorescent analysis of Hela cells, using GTF2I antibody 10499-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman thyroid cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue, mouse small intestine tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10499-1-AP targets TFII I in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFII I fusion protein Ag0773
Full Name general transcription factor II, i
Calculated Molecular Weight 112 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC004472
Gene Symbol TFII I
Gene ID (NCBI) 2969
RRIDAB_2114813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].

Protocols

Product Specific Protocols
WB protocol for TFII I antibody 10499-1-APDownload protocol
IHC protocol for TFII I antibody 10499-1-APDownload protocol
IF protocol for TFII I antibody 10499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer

Authors - Jing Chen
View Article
mouseWB

Nature

Post-translational control of beige fat biogenesis by PRDM16 stabilization

Authors - Qiang Wang
View Article
mouseIF

iScience

Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain

Authors - Terry R Suk
View Article