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GTF2H2 Polyclonal antibody

GTF2H2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16005-1-AP

Synonyms

BTF2, BTF2 p44, BTF2P44, GTF2H2, T BTF2P44, TFIIH

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using GTF2H2 Polyclonal antibody (16005-1-AP)

    WB analysis of K-562 using 16005-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GTF2H2 Polyclonal antibody (16005-1-AP)

    WB analysis of K-562 using 16005-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GTF2H2 Polyclonal antibody (16005-1-AP)

    WB analysis of HepG2 using 16005-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GTF2H2 Polyclonal antibody (16005-1-AP)

    WB analysis of K-562 using 16005-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16005-1-AP targets GTF2H2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2H2 fusion protein Ag8653
Full Name general transcription factor IIH, polypeptide 2, 44kDa
Calculated Molecular Weight395aa,44 kDa; 165aa,19 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC005345
Gene Symbol GTF2H2
Gene ID (NCBI) 2966
RRIDAB_2279415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor IIH(TFIIH) is associated with the RNA polymerase II transcription complex, which is involved in transcription and transcription-mediated DNA repair. GTF2H2(GENERAL TRANSCRIPTION FACTOR IIH, POLYPEPTIDE 2) is one of the six subnuits forming the core-TFIIH basal transcriptional factor. TFIIH plays a role in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N terminus of GTF2H2 interacts with and regulates XPD, and an intact C terminus is required for a successful escape of RNAPol II from the promoter. This is a rabbit polyclonal antibody producted by part of N-terminal GTF2H2 of human origin.

Protocols

Product Specific Protocols
WB protocol for GTF2H2 antibody 16005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.

Authors - Ashleigh E Schaffer
View Article
humanWB

Tissue Cell

Exosome-derived GTF2H2 from Huh7 cells can inhibit endothelial cell viability, migration, tube formation, and permeability

Authors - Zhenkun Li
View Article