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GSTT2 Polyclonal antibody

GSTT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15063-1-AP

Synonyms

GSTT2B, GST class-theta-2, GST class theta 2, Glutathione S-transferase theta-2, EC:2.5.1.18

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of rat liver using 15063-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of mouse liver using 15063-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of L02 using 15063-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of mouse lung using 15063-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    IHC staining of human prostate cancer using 15063-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15063-1-AP (GSTT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    IHC staining of human prostate cancer using 15063-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15063-1-AP (GSTT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using GSTT2 Polyclonal antibody (15063-1-AP)

    IF Staining of L02 using 15063-1-AP

    Immunofluorescent analysis of L02 cells using 15063-1-AP (GSTT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inrat liver tissue, L02 cells, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15063-1-AP targets GSTT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTT2 fusion protein Ag7066
Full Name glutathione S-transferase theta 2
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC002415
Gene Symbol GSTT2
Gene ID (NCBI) 2953
RRIDAB_2878106
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSTT2 antibody 15063-1-APDownload protocol
IHC protocol for GSTT2 antibody 15063-1-APDownload protocol
IF protocol for GSTT2 antibody 15063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Bioorg Chem

Two resveratrol analogs, pinosylvin and 4,4'-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway.

Authors - Cheng-Niu Wang
View Article