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GSTO1 Monoclonal antibody

GSTO1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5E1E10

Cat no : 60150-2-Ig

Synonyms

glutathione S transferase omega 1, GSTO 1, GSTO1, P28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis using 60150-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of human heart using 60150-2-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of U-937 using 60150-2-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of Raji using 60150-2-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of ROS1728 using 60150-2-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of NIH/3T3 using 60150-2-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human oesophagus cancer using 60150-2-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human oesophagus cancer using 60150-2-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver cancer using 60150-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver cancer using 60150-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver using 60150-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver using 60150-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    IF Staining of PC-3 using 60150-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GSTO1 antibody (60150-2-Ig, Clone: 5E1E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, human heart tissue, U-937 cells, Raji cells, ROS1728 cells, NIH/3T3 cells, A431 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman oesophagus cancer tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60150-2-Ig targets GSTO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSTO1 fusion protein Ag7199
Full Name glutathione S-transferase omega 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC000127
Gene Symbol GSTO1
Gene ID (NCBI) 9446
RRIDAB_10644447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells.

Protocols

Product Specific Protocols
WB protocol for GSTO1 antibody 60150-2-IgDownload protocol
IHC protocol for GSTO1 antibody 60150-2-IgDownload protocol
IF protocol for GSTO1 antibody 60150-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Toxicol Appl Pharmacol

Effects of Nrf2 deficiency on arsenic metabolism in mice.

Authors - Huihui Wang
View Article