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  • Featured Product
  • KD/KO Validated

GSDMD Polyclonal antibody

GSDMD Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 20770-1-AP

Synonyms

Gasdermin-D, C-terminal, Gasdermin-D, gasderminD, Gasdermin domain-containing protein 1, gasdermin D

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GSDMD Polyclonal antibody (20770-1-AP)

    WB analysis of Jurkat using 20770-1-AP

    WB result of GSDMD antibody (20770-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSDMD transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using GSDMD Polyclonal antibody (20770-1-AP)

    WB analysis using 20770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GSDMD Polyclonal antibody (20770-1-AP)

    WB analysis using 20770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of THP-1 cells using GSDMD Polyclonal antibody (20770-1-AP)

    IP experiment of THP-1 using 20770-1-AP

    IP result of anti-GSDMD (IP:20770-1-AP, 4ug; Detection:20770-1-AP 1:4000) with THP-1 cells lysate 1560 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GSDMD Polyclonal antibody (20770-1-AP)

    IHC staining of human ovary cancer using 20770-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GSDMD Polyclonal antibody (20770-1-AP)

    IHC staining of human ovary cancer using 20770-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSDMD Polyclonal antibody (20770-1-AP)

    IF Staining of HeLa using 20770-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSDMD Polyclonal antibody (20770-1-AP)

    IF Staining of HeLa using 20770-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Jurkat cells using GSDMD Polyclonal antibody (20770-1-AP)

    FC experiment of Jurkat using 20770-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug GSDMD Polyclonal antibody (20770-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, NIH/3T3 cells, Jurkat cells, C6 cells, rat liver tissue, rat brain tissue
Positive IP detected inTHP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20770-1-AP targets GSDMD in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSDMD fusion protein Ag14757
Full Name gasdermin D
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight 50-53 kDa
GenBank Accession NumberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_10696319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
WB protocol for GSDMD antibody 20770-1-APDownload protocol
IHC protocol for GSDMD antibody 20770-1-APDownload protocol
IF protocol for GSDMD antibody 20770-1-APDownload protocol
IP protocol for GSDMD antibody 20770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

Authors - Xing Liu
  • KD Validated
View Article
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
View Article
humanWB

Acta Pharm Sin B

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

Authors - Xiaowei Zhang
View Article
humanWB

Acta Pharm Sin B

A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer

Authors - Yang Liu
View Article
HumanWB

Cell Res

Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion.

Authors - Wan-Ting He
View Article
humanWB

J Exp Med

Protein kinase D at the Golgi controls NLRP3 inflammasome activation.

Authors - Zhirong Zhang
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Isha (Verified Customer) (03-02-2021)

Bands were so clear. Could not refrain myself from writing this review.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mice kidney