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GPSM1 Polyclonal antibody

GPSM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11483-1-AP

Synonyms

G-protein-signaling modulator 1, AGS3, Activator of G-protein signaling 3



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11483-1-AP targets GPSM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPSM1 fusion protein Ag2018
Full Name G-protein signaling modulator 1 (AGS3-like, C. elegans)
Calculated Molecular Weight 652 aa, 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC017353
Gene Symbol GPSM1
Gene ID (NCBI) 26086
RRIDAB_2279358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).

Protocols

Product Specific Protocols
WB protocol for GPSM1 antibody 11483-1-APDownload protocol
IHC protocol for GPSM1 antibody 11483-1-APDownload protocol
IF protocol for GPSM1 antibody 11483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Nat Commun

GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages

Authors - Jing Yan
ratWB,IHC

J Ovarian Res

Depletion of GPSM1 enhances ovarian granulosa cell apoptosis via cAMP-PKA-CREB pathway in vitro.

Authors - Xuzi Cai
  • KD Validated
mouseWB,IF

Mol Metab

GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis and provokes diet-induced obesity

Authors - Mengyang Tang