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  • Featured Product
  • KD/KO Validated

Geminin Polyclonal antibody

Geminin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10802-1-AP

Synonyms

GMNN, MGORS6, Gem

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Geminin Polyclonal antibody (10802-1-AP)

    WB analysis using 10802-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Geminin Polyclonal antibody (10802-1-AP)

    WB analysis of human testis using 10802-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Geminin Polyclonal antibody (10802-1-AP)

    WB analysis of HEK-293 using 10802-1-AP

    WB result of Geminin antibody (10802-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Geminin transfected HEK-293 cells.

  • Western Blot (WB) analysis of mouse testis tissue using Geminin Polyclonal antibody (10802-1-AP)

    WB analysis of mouse testis using 10802-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Geminin Polyclonal antibody (10802-1-AP)

    WB analysis of HEK-293 using 10802-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Geminin Polyclonal antibody (10802-1-AP)

    IP experiment of HEK-293 using 10802-1-AP

    IP result of anti-Geminin (IP:10802-1-AP, 4ug; Detection:10802-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Geminin Polyclonal antibody (10802-1-AP)

    IHC staining of human colon cancer using 10802-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Geminin Polyclonal antibody (10802-1-AP)

    IHC staining of human colon cancer using 10802-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Geminin Polyclonal antibody (10802-1-AP)

    IHC staining of human colon cancer using 10802-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Geminin Polyclonal antibody (10802-1-AP)

    IHC staining of human colon cancer using 10802-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Geminin Polyclonal antibody (10802-1-AP)

    IHC staining of human breast cancer using 10802-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10802-1-AP (Geminin antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Geminin Polyclonal antibody (10802-1-AP)

    IF Staining of HepG2 using 10802-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Geminin antibody (10802-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using Geminin Polyclonal antibody (10802-1-AP)

    FC experiment of HeLa using 10802-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Geminin (10802-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using Geminin Polyclonal antibody (10802-1-AP)

    FC experiment of HeLa using 10802-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Geminin (10802-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inHEK-293 cells, human testis tissue, mouse testis tissue, HeLa cells; mouse testis tissue, rat testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10802-1-AP targets Geminin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Geminin fusion protein Ag1076
Full Name geminin, DNA replication inhibitor
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 28-29 kDa
GenBank Accession NumberBC005185
Gene Symbol GMNN
Gene ID (NCBI) 51053
RRIDAB_2110945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)

Protocols

Product Specific Protocols
WB protocol for Geminin antibody 10802-1-APDownload protocol
IHC protocol for Geminin antibody 10802-1-APDownload protocol
IF protocol for Geminin antibody 10802-1-APDownload protocol
IP protocol for Geminin antibody 10802-1-APDownload protocol
FC protocol for Geminin antibody 10802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Whole genomes redefine the mutational landscape of pancreatic cancer.

Authors - Nicola Waddell
View Article
humanIF

Cancer Discov

Biomarkers Associating with PARP Inhibitor Benefit in Prostate Cancer in the TOPARP-B Trial.

Authors - Suzanne Carreira
View Article
humanIF

Ann Oncol

Association of RAD51 with homologous recombination deficiency (HRD) and clinical outcomes in untreated triple-negative breast cancer (TNBC): analysis of the GeparSixto randomized clinical trial.

Authors - A Llop-Guevara
View Article
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
View Article
mouseIF

Nat Commun

Homologous recombination deficiency derived from whole-genome sequencing predicts platinum response in triple-negative breast cancers

Authors - Petra Ter Brugge
View Article
humanIF

Sci Transl Med

BRCAness, SLFN11, and RB1 loss predict response to topoisomerase I inhibitors in triple-negative breast cancers.

Authors - Florence Coussy
View Article