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KGA/GAC Monoclonal antibody
KGA/GAC Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
3A12A1
Cat no : 66265-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, rat liver tissue, fetal human brain tissue, U-251 cells, HEK-293 cells |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | L02 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
66265-1-Ig targets KGA/GAC in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KGA/GAC fusion protein Ag24227 |
| Full Name | glutaminase |
| Calculated Molecular Weight | 669 aa, 73 kDa |
| Observed Molecular Weight | 58 kDa, 65 kDa |
| GenBank Accession Number | BC038507 |
| Gene Symbol | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_2881651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KGA/GAC antibody 66265-1-Ig | Download protocol |
| IHC protocol for KGA/GAC antibody 66265-1-Ig | Download protocol |
| IF protocol for KGA/GAC antibody 66265-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
Cell Rep Glutaminase 1 deficiency confined in forebrain neurons causes autism spectrum disorder-like behaviors | ||
Oxid Med Cell Longev Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages. | ||
Int J Oncol Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah-Eve (Verified Customer) (06-02-2023) | Works well
|
FH Longlong (Verified Customer) (03-01-2022) | I'm working on a glutaminase project. Great western blot results and fantastic consistency
 |
