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  • Featured Product
  • KD/KO Validated

GFM2 Polyclonal antibody

GFM2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16941-1-AP

Synonyms

Elongation factor G2, EF-G2mt, EFG2mt, EFG2, EF G2mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GFM2 Polyclonal antibody (16941-1-AP)

    WB analysis using 16941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16941-1-AP (GFM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GFM2 Polyclonal antibody (16941-1-AP)

    IP experiment of HepG2 using 16941-1-AP

    IP result of anti-GFM2 (IP:16941-1-AP, 4ug; Detection:16941-1-AP 1:500) with HepG2 cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GFM2 Polyclonal antibody (16941-1-AP)

    IF Staining of HepG2 using 16941-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16941-1-AP (GFM2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16941-1-AP targets GFM2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFM2 fusion protein Ag10530
Full Name G elongation factor, mitochondrial 2
Calculated Molecular Weight 779 aa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC015712
Gene Symbol GFM2
Gene ID (NCBI) 84340
RRIDAB_2110151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GFM2 antibody 16941-1-APDownload protocol
IF protocol for GFM2 antibody 16941-1-APDownload protocol
IP protocol for GFM2 antibody 16941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.

Authors - Takumi Yokokawa
View Article
humanWB

Nat Metab

Tetracyclines promote survival and fitness in mitochondrial disease models.

Authors - Elizabeth A Perry
  • KO Validated
View Article
mouseWB

Physiol Rep

Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.

Authors - Takumi Yokokawa
View Article

EMBO J

Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism

Authors - Ying Shu
View Article