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GEMIN8 Polyclonal antibody

GEMIN8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15251-1-AP

Synonyms

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GEMIN8 Polyclonal antibody (15251-1-AP)

    WB analysis using 15251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GEMIN8 Polyclonal antibody (15251-1-AP)

    WB analysis of HEK-293 using 15251-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GEMIN8 Polyclonal antibody (15251-1-AP)

    IP experiment of HepG2 using 15251-1-AP

    IP result of anti-GEMIN8 (IP:15251-1-AP, 3ug; Detection:15251-1-AP 1:400) with HepG2 cells lysate 1720ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GEMIN8 Polyclonal antibody (15251-1-AP)

    IHC staining of human liver cancer using 15251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GEMIN8 Polyclonal antibody (15251-1-AP)

    IHC staining of human liver cancer using 15251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GEMIN8 Polyclonal antibody (15251-1-AP)

    IF Staining of HepG2 using 15251-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15251-1-AP targets GEMIN8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GEMIN8 fusion protein Ag6774
Full Name gem (nuclear organelle) associated protein 8
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC003607
Gene Symbol GEMIN8
Gene ID (NCBI) 54960
RRIDAB_2111845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.

Protocols

Product Specific Protocols
WB protocol for GEMIN8 antibody 15251-1-APDownload protocol
IHC protocol for GEMIN8 antibody 15251-1-APDownload protocol
IF protocol for GEMIN8 antibody 15251-1-APDownload protocol
IP protocol for GEMIN8 antibody 15251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Authors - Anne-Sophie Gribling-Burrer
View Article