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GEFT Polyclonal antibody

GEFT Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17028-1-AP

Synonyms

ARHGEF25, GEFT, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using GEFT Polyclonal antibody (17028-1-AP)

    WB analysis of human brain using 17028-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17028-1-AP (GEFT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using GEFT Polyclonal antibody (17028-1-AP)

    IP experiment of HEK-293 using 17028-1-AP

    IP result of anti-GEFT (IP:17028-1-AP, 4ug; Detection:17028-1-AP 1:400) with HEK-293 cells lysate 2000ug.

Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17028-1-AP targets GEFT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GEFT fusion protein Ag10436
Full Name RhoA/RAC/CDC42 exchange factor
Calculated Molecular Weight64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC012860
Gene Symbol GEFT
Gene ID (NCBI) 115557
RRIDAB_2279083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).

Protocols

Product Specific Protocols
WB protocol for GEFT antibody 17028-1-APDownload protocol
IP protocol for GEFT antibody 17028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols