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GCOM1 Polyclonal antibody

GCOM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18129-1-AP

Synonyms

GRINL1A upstream protein, GRINL1A complex locus, GRINL1A combined protein, GRINL1A, Gcom2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCOM1 Polyclonal antibody (18129-1-AP)

    WB analysis using 18129-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18129-1-AP (GCOM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCOM1 Polyclonal antibody (18129-1-AP)

    IHC staining of human liver cancer using 18129-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18129-1-AP (GCOM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCOM1 Polyclonal antibody (18129-1-AP)

    IHC staining of human liver cancer using 18129-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18129-1-AP (GCOM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GCOM1 Polyclonal antibody (18129-1-AP)

    IF Staining of HepG2 using 18129-1-AP

    Immunofluorescent analysis of HepG2 cells, using GCOM1 antibody 18129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18129-1-AP targets GCOM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCOM1 fusion protein Ag12708
Full Name GRINL1A complex locus
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight 64 kDa, 44-54 kDa
GenBank Accession NumberBC101645
Gene Symbol GCOM1
Gene ID (NCBI) 145781
RRIDAB_2232101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCOM1, also named as Myozap, Gup and Gcom, belongs to the GCOM1 family. It plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF. GCOM1 has 11 isoforms with MW 44-54kd and 64kd.

Protocols

Product Specific Protocols
WB protocol for GCOM1 antibody 18129-1-APDownload protocol
IHC protocol for GCOM1 antibody 18129-1-APDownload protocol
IF protocol for GCOM1 antibody 18129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols