GCDH Polyclonal antibody
GCDH Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14930-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
14930-1-AP targets GCDH in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GCDH fusion protein Ag6713 |
| Full Name | glutaryl-Coenzyme A dehydrogenase |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 44-48 kDa |
| GenBank Accession Number | BC002579 |
| Gene Symbol | GCDH |
| Gene ID (NCBI) | 2639 |
| RRID | AB_2878092 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCDH is a nuclear-encoded mitochondrial protein involved in the catabolic pathway of tryptophan, lysine and hydroxylysine. The functional enzyme is a homotetramer of 43.3 kDa subunits that resides in the mitochondrial matrix. GCDH activity reduction leads to predominant accumulation of glutaric (GA) and 3- hydroxyglutaric (3HGA) acids in tissues, mostly brain, and biological fluids of affected patients. GCDH is widely expressed and has the highest levels in liver and kidney, which is consistent with its role in amino acid oxidation. (PMID: 27984186, 33001399)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GCDH antibody 14930-1-AP | Download protocol |
| IHC protocol for GCDH antibody 14930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage. | ||
PLoS One Effects of targeted suppression of glutaryl-CoA dehydrogenase by lentivirus-mediated shRNA and excessive intake of lysine on apoptosis in rat striatal neurons. | ||
Mol Metab Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation. | ||
Front Pharmacol Proteomic analysis revealed the pharmacological mechanism of Xueshuantong injection in preventing early acute myocardial infarction injury |
