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GCAT Polyclonal antibody

GCAT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16317-1-AP

Synonyms

AKB ligase, Aminoacetone synthase, GCAT, Glycine acetyltransferase, KBL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCAT Polyclonal antibody (16317-1-AP)

    WB analysis using 16317-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16317-1-AP (GCAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using GCAT Polyclonal antibody (16317-1-AP)

    WB analysis of mouse pancreas using 16317-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16317-1-AP (GCAT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using GCAT Polyclonal antibody (16317-1-AP)

    IHC staining of human pancreas using 16317-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 16317-1-AP (GCAT antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using GCAT Polyclonal antibody (16317-1-AP)

    IHC staining of human pancreas using 16317-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 16317-1-AP (GCAT antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16317-1-AP targets GCAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCAT fusion protein Ag9367
Full Name glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase)
Calculated Molecular Weight 419 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC014457
Gene Symbol GCAT
Gene ID (NCBI) 23464
RRIDAB_2263046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GCAT antibody 16317-1-APDownload protocol
IHC protocol for GCAT antibody 16317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Life Sci Alliance

Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.

Authors - Shengya Tian
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Lana (Verified Customer) (10-04-2019)

SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 cells. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: ST-Hdh mouse striatal cell line
GCAT Antibody Western Blot, validation (1:2000 dilution) in ST-Hdh mouse striatal cell line (Cat no:16317-1-AP)