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GBP6 Polyclonal antibody

GBP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23001-1-AP

Synonyms

Guanylate-binding protein 6, Guanine nucleotide-binding protein 6, GTP-binding protein 6, GBP-6, EC:3.6.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GBP6 Polyclonal antibody (23001-1-AP)

    WB analysis of HeLa using 23001-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23001-1-AP (GBP6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using GBP6 Polyclonal antibody (23001-1-AP)

    IHC staining of human testis using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23001-1-AP (GBP6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GBP6 Polyclonal antibody (23001-1-AP)

    IHC staining of human testis using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23001-1-AP (GBP6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GBP6 Polyclonal antibody (23001-1-AP)

    IHC staining of human lung cancer using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23001-1-AP (GBP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

23001-1-AP targets GBP6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP6 fusion protein Ag19196
Full Name guanylate binding protein family, member 6
Calculated Molecular Weight 633 aa, 72 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC131713
Gene Symbol GBP6
Gene ID (NCBI) 163351
RRIDAB_2879195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate-binding protein 6 (GBP6) is a member of the guanylate-binding protein family and is best known as a gene induced by interferon-γ signalling. Interferon-γ is produced predominantly by NK and T-cells, indicating that GBP6 is involved in host immune response (PMID: 28747659, 27029383). GBP6 has 2 isoforms with the molecular mass of 34 and 72 kDa.

Protocols

Product Specific Protocols
WB protocol for GBP6 antibody 23001-1-APDownload protocol
IHC protocol for GBP6 antibody 23001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article
humanWB

Clin Oral Investig

Guanylate-binding protein 6 is a novel biomarker for tumorigenesis and prognosis in tongue squamous cell carcinoma.

Authors - Pei-Feng Liu
View Article