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GATC Polyclonal antibody

GATC Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23183-1-AP

Synonyms

15E1.2, GATC, GatC like protein, Protein 15E1.2

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse liver tissue using GATC Polyclonal antibody (23183-1-AP)

    IP experiment of mouse liver using 23183-1-AP

    IP result of anti-GATC (IP:23183-1-AP, 3ug; Detection:23183-1-AP 1:500) with mouse liver tissue lysate 12500ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using GATC Polyclonal antibody (23183-1-AP)

    IHC staining of human liver using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using GATC Polyclonal antibody (23183-1-AP)

    IHC staining of human liver using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using GATC Polyclonal antibody (23183-1-AP)

    IHC staining of human appendicitis using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using GATC Polyclonal antibody (23183-1-AP)

    IHC staining of human appendicitis using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:400 (under 40x lens).

Tested Applications

Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23183-1-AP targets GATC in IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATC fusion protein Ag19713
Full Name glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
Calculated Molecular Weight 136 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC107145
Gene Symbol GATC
Gene ID (NCBI) 283459
RRIDAB_2879226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).

Protocols

Product Specific Protocols
IHC protocol for GATC antibody 23183-1-APDownload protocol
IP protocol for GATC antibody 23183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols