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GALE Polyclonal antibody

GALE Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14414-1-AP

Synonyms

UDP-GlcNAc 4-epimerase, UDP-GalNAc 4-epimerase, UDP-galactose 4-epimerase, SDR1E1, EC:5.1.3.7



Tested Applications

Positive WB detected inA375 cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14414-1-AP targets GALE in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALE fusion protein Ag5756
Full Name UDP-galactose-4-epimerase
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC050685
Gene Symbol GALE
Gene ID (NCBI) 2582
RRIDAB_2108540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).

Protocols

Product Specific Protocols
WB protocol for GALE antibody 14414-1-APDownload protocol
IHC protocol for GALE antibody 14414-1-APDownload protocol
IF protocol for GALE antibody 14414-1-APDownload protocol
IP protocol for GALE antibody 14414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Redox Biol

Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Authors - Wei Ou
humanWB, IHC

PLoS One

Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.

Authors - Yinxian Wen
humanWB

Infect Drug Resist

iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae.

Authors - Yan Wang
humanWB, IHC

Biochem Biophys Res Commun

The critical role of UDP-galactose-4-epimerase in osteoarthritis: modulating proteoglycans synthesis of articular chondrocytes.

Authors - Yinxian Wen
  • KD Validated