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GALC Polyclonal antibody

GALC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12887-1-AP

Synonyms

Galactocerebrosidase, galactosylceramidase, GALC, GALCERase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A375 cells using GALC Polyclonal antibody (12887-1-AP)

    WB analysis of A375 using 12887-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GALC Polyclonal antibody (12887-1-AP)

    WB analysis of HeLa using 12887-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using GALC Polyclonal antibody (12887-1-AP)

    WB analysis of human placenta using 12887-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GALC Polyclonal antibody (12887-1-AP)

    WB analysis of mouse brain using 12887-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA375 cells, HeLa cells, human placenta tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

12887-1-AP targets GALC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALC fusion protein Ag3915
Full Name galactosylceramidase
Calculated Molecular Weight 668 aa, 76 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC036518
Gene Symbol GALC
Gene ID (NCBI) 2581
RRIDAB_2294473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galactosylceramidase (Galc) is a lysosomal enzyme involved in the catabolism of galactosylceramide, a major lipid in myelin, kidney, and epithelial cells of the small intestine and colon. The normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. Mutation of GALC gene will lead to Krabbe disease (globoid cell leukodystrophy), an autosomal recessive neurodegenerative disorder that affects both the central and peripheral nervous system due to an enzymatic defect of galactocerebrosidase.

Protocols

Product Specific Protocols
WB protocol for GALC antibody 12887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Chembiochem

A specific activity-based probe to monitor family GH59 galactosylceramidase - the enzyme deficient in Krabbe disease.

Authors - André Ra Marques
View Article