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GABPB1 Polyclonal antibody

Name: GABPB1 Polyclonal antibody
Brand: Proteintech
SKU: 12597-1-AP

GABPB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12597-1-AP

Synonyms

GA-binding protein subunit beta-1, E4TF1B, E4TF1 53, E4TF1 47, E4TF1

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of mouse lung using 12597-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12597-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12597-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12597-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12597-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12597-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12597-1-AP
IP result of anti-GABPB1 (IP:12597-1-AP, 4ug; Detection:12597-1-AP 1:1000) with HeLa cells lysate 520ug.
IHC staining of human testis using 12597-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 12597-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 12597-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 12597-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 12597-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12597-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 12597-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPB1 antibody (12597-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 12597-1-AP

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Tested Applications

Positive WB detected in	A375 cells, MCF-7 cells, mouse lung tissue, HeLa cells, HepG2 cells, mouse testis tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

12597-1-AP targets GABPB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GABPB1 fusion protein Ag3208
Full Name	GA binding protein transcription factor, beta subunit 1
Calculated Molecular Weight	347 aa, 38 kDa
Observed Molecular Weight	36-45 kDa, 47-53 kDa
GenBank Accession Number	BC016910
Gene Symbol	GABPB1
Gene ID (NCBI)	2553
RRID	AB_10951115
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABPB1, also named as E4TF1B, GABPB, GABPB2, E4TF1-47 and E4TF1-53, is a transcription factor capable of interacting with purine rich repeats (GA repeats). It is necessary for the expression of the Adenovirus E4 gene. GABPB1 has 4 isoforms. The MW-of Beta 1 is 53kd and Beta-2 is 47kd(PMID:9525935). This antibody can recognize all the isoforms.

Protocols

Product Specific Protocols
WB protocol for GABPB1 antibody 12597-1-AP	Download protocol
IHC protocol for GABPB1 antibody 12597-1-AP	Download protocol
IF protocol for GABPB1 antibody 12597-1-AP	Download protocol
IP protocol for GABPB1 antibody 12597-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner. Authors - Andrew Mancini KD Validated View Article
human	WB	Proc Natl Acad Sci U S A Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage. Authors - Alexandra M Amen View Article
human, mouse	WB,IHC,IF	Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Authors - Wu Hongtan H KD Validated View Article
human	WB	Sci Rep LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells. Authors - Wenchuan Qi KD Validated View Article
human	WB	Biology (Basel) TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis. Authors - Yanbing Wang View Article
mouse	WB	Brain Res Bull ZFP36 protects against oxygen-glucose deprivation/reoxygenation-induced mitochondrial fragmentation and neuronal apoptosis through inhibiting NOX4-DRP1 pathway. Authors - Hengjiang Guo View Article

GABPB1 Polyclonal antibody

GABPB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse lung using 12597-1-AP

WB analysis using 12597-1-AP

WB analysis of HeLa using 12597-1-AP

WB analysis of HepG2 using 12597-1-AP

WB analysis of mouse testis using 12597-1-AP

WB analysis of MCF-7 using 12597-1-AP

IP experiment of HeLa using 12597-1-AP

IHC staining of human testis using 12597-1-AP

IHC staining of human testis using 12597-1-AP

IHC staining of human testis using 12597-1-AP

IHC staining of human testis using 12597-1-AP

IHC staining of human testis using 12597-1-AP

IHC staining of human testis using 12597-1-AP

IF Staining of HeLa using 12597-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.

Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.

ZFP36 protects against oxygen-glucose deprivation/reoxygenation-induced mitochondrial fragmentation and neuronal apoptosis through inhibiting NOX4-DRP1 pathway.