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GABARAP Polyclonal antibody

Name: GABARAP Polyclonal antibody
Brand: Proteintech
SKU: 18723-1-AP
Rating: 3.67 (3 reviews)

GABARAP Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (3)

Cat no : 18723-1-AP

Synonyms

MM46, HT004, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, FLC3B

Validation Data Gallery

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AllWBIF/ICCFC (Intra)

WB analysis using 18723-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 18723-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 18723-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 18723-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 18723-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 18723-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 18723-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 18723-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 18723-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells and untreated HepG2 cells using GABARAP antibody (18723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 18723-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells and untreated HepG2 cells using 18723-1-AP (GABARAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 18723-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GABARAP (18723-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 18723-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue
Positive IF/ICC detected in	HepG2 cells, Starvation treated HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 18 publications below
IF	See 4 publications below
CoIP	See 5 publications below

Product Information

18723-1-AP targets GABARAP in WB, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Full Name	GABA(A) receptor-associated protein
Calculated Molecular Weight	14 kDa
Observed Molecular Weight	14-18 kDa
GenBank Accession Number	BC106748
Gene Symbol	GABARAP
Gene ID (NCBI)	11337
RRID	AB_10603365
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Protocols

Product Specific Protocols
WB protocol for GABARAP antibody 18723-1-AP	Download protocol
IF protocol for GABARAP antibody 18723-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. Authors - Linyu Sun View Article
human	WB	Autophagy ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis Authors - Jinjin Zhang KD Validated View Article
human	WB	Autophagy ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1 Authors - Weihong Xie KO Validated View Article
	IF	Nat Commun PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection. Authors - Shifan Wang View Article
human	WB	Autophagy ATF4 links ER stress with reticulophagy in glioblastoma cells. Authors - Svenja Zielke View Article
mouse	WB	Autophagy ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8. Authors - Mads Skytte Rasmussen View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Hala (Verified Customer) (05-09-2023)	works very good in milk 5% Applications: Western Blot Primary Antibody Dilution: 1/3000 Cell Tissue Type: HepG2 GABARAP Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:18723-1-AP)
FH Hala (Verified Customer) (04-28-2023)	works very good with milk blocking Applications: Western Blot Primary Antibody Dilution: 1/3000 Cell Tissue Type: HepG2
FH Anita (Verified Customer) (12-22-2020)	HeLa cell protein extract (5 micrograms) Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: HeLa cells GABARAP Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:18723-1-AP)

GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 18723-1-AP

WB analysis of A549 using 18723-1-AP

WB analysis of mouse skeletal muscle using 18723-1-AP

WB analysis of mouse brain using 18723-1-AP

WB analysis of human placenta using 18723-1-AP

WB analysis of mouse pancreas using 18723-1-AP

WB analysis of human heart using 18723-1-AP

WB analysis using 18723-1-AP

IF Staining of HepG2 using 18723-1-AP

IF Staining of HepG2 using 18723-1-AP

FC experiment of HepG2 using 18723-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.

ATF4 links ER stress with reticulophagy in glioblastoma cells.

ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.

Reviews