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  • KD/KO Validated

GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18723-1-AP

Synonyms

MM46, HT004, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, FLC3B



Tested Applications

Positive WB detected inHeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells, Starvation treated HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18723-1-AP targets GABARAP in WB, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GABA(A) receptor-associated protein
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-18 kDa
GenBank Accession NumberBC106748
Gene Symbol GABARAP
Gene ID (NCBI) 11337
RRIDAB_10603365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Protocols

Product Specific Protocols
WB protocol for GABARAP antibody 18723-1-APDownload protocol
IF protocol for GABARAP antibody 18723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
humanWB

Autophagy

ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis

Authors - Jinjin Zhang
  • KD Validated
humanWB

Autophagy

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

Authors - Weihong Xie
  • KO Validated
IF

Nat Commun

PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.

Authors - Shifan Wang
humanWB

Autophagy

ATF4 links ER stress with reticulophagy in glioblastoma cells.

Authors - Svenja Zielke
mouseWB

Autophagy

ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.

Authors - Mads Skytte Rasmussen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hala (Verified Customer) (05-09-2023)

works very good in milk 5%

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/3000
  • Cell Tissue Type: HepG2
GABARAP Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:18723-1-AP)
FH

Hala (Verified Customer) (04-28-2023)

works very good with milk blocking

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/3000
  • Cell Tissue Type: HepG2
FH

Anita (Verified Customer) (12-22-2020)

HeLa cell protein extract (5 micrograms)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
GABARAP Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:18723-1-AP)