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GAB3-Specific Polyclonal antibody

GAB3-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20089-1-AP

Synonyms

GAB3, Growth factor receptor bound protein 2-associated protein 3, GRB2-associated-binding protein 3, GRB2-associated binder 3, GRB2 associated binder 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GAB3-Specific Polyclonal antibody (20089-1-AP)

    WB analysis of mouse brain using 20089-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GAB3-Specific Polyclonal antibody (20089-1-AP)

    WB analysis of HepG2 using 20089-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GAB3-Specific Polyclonal antibody (20089-1-AP)

    WB analysis of MCF-7 using 20089-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using GAB3-Specific Polyclonal antibody (20089-1-AP)

    IP experiment of MCF-7 using 20089-1-AP

    IP result of anti-GAB3-Specific (IP:20089-1-AP, 4ug; Detection:20089-1-AP 1:2000) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAB3-Specific Polyclonal antibody (20089-1-AP)

    IHC staining of human liver cancer using 20089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAB3-Specific Polyclonal antibody (20089-1-AP)

    IHC staining of human liver cancer using 20089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GAB3-Specific Polyclonal antibody (20089-1-AP)

    IF Staining of MCF-7 using 20089-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20089-1-AP (GAB3-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20089-1-AP targets GAB3-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GRB2-associated binding protein 3
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberNM_001081573
Gene Symbol GAB3
Gene ID (NCBI) 139716
RRIDAB_11182715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAB3 belongs to the GAB family. GAB3 interacts with PIK3R/p85, SHP2 and GRAP2/MONA. It may interact with Grb2. The antibody is specific to GAB3.

Protocols

Product Specific Protocols
WB protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IHC protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IF protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IP protocol for GAB3-Specific antibody 20089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols