GAB3-Specific Polyclonal antibody

GAB3-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20089-1-AP

Synonyms

GAB3, Growth factor receptor bound protein 2-associated protein 3, GRB2-associated-binding protein 3, GRB2-associated binder 3, GRB2 associated binder 3



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20089-1-AP targets GAB3-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GRB2-associated binding protein 3
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberNM_001081573
Gene Symbol GAB3
Gene ID (NCBI) 139716
RRIDAB_11182715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAB3 belongs to the GAB family. GAB3 interacts with PIK3R/p85, SHP2 and GRAP2/MONA. It may interact with Grb2. The antibody is specific to GAB3.

Protocols

Product Specific Protocols
WB protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IHC protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IF protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IP protocol for GAB3-Specific antibody 20089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols