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GAB2 Polyclonal antibody

GAB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22549-1-AP

Synonyms

GAB2, GRB2 associated binder 2, KIAA0571, pp100



Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22549-1-AP targets GAB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAB2 fusion protein Ag18192
Full Name GRB2-associated binding protein 2
Calculated Molecular Weight 676 aa, 74 kDa
Observed Molecular Weight 74-90 kDa
GenBank Accession NumberBC131711
Gene Symbol GAB2
Gene ID (NCBI) 9846
RRIDAB_2879119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GAB2 antibody 22549-1-APDownload protocol
IHC protocol for GAB2 antibody 22549-1-APDownload protocol
IF protocol for GAB2 antibody 22549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
humanWB

Am J Transl Res

Silencing of lncRNA HOXA11-AS inhibits cell migration, invasion, proliferation, and promotes apoptosis in human glioma cells via upregulating microRNA-125a: in vitro and in vivo studies.

Authors - Jianxin Jiang
mouseIHC

Oncol Rep

MicroRNA‑218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2.

Authors - Lijun Mu
humanWB

Front Physiol

Enhanced Autophagy in GAB1-Deficient Vascular Endothelial Cells Is Responsible for Atherosclerosis Progression.

Authors - Xin Qian
human,mouseWB,IF

Int J Mol Med

Gab2 promotes the growth of colorectal cancer by regulating the M2 polarization of tumor‑associated macrophages

Authors - Xuehan Gao