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GA repeat Polyclonal antibody

GA repeat Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, Dot Blot, ELISA

Conjugate

Unconjugated

Cat no : 24492-1-AP

Synonyms

C9RAN, GA repeat, GAGAGAGAGA, GGGCC, poly GA

Validation Data Gallery

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  • ELISA experiment of peptide using GA repeat Polyclonal antibody (24492-1-AP)

    ELISA experiment of peptide using 24492-1-AP

    GA repeat Antibody (24492-1-AP) tested in ELISA. GA repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of GA repeat antibody (24492-1-AP) . Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Tested Applications

Positive ELISA detected inpeptide

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24492-1-AP targets GA repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GA repeat
Gene Symbol
Gene ID (NCBI)
RRIDAB_2879571
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "GA repeat" sequence. This antibody detects the GAGAGAGAGA peptide with dilution 1:32,000 in Elisa.

Publications

SpeciesApplicationTitle

Nat Neurosci

Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.

Authors - Kornélia Szebényi
View Article
mouseIHC

EMBO Mol Med

Active poly-GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model.

Authors - Qihui Zhou
View Article
humanWB, IHC

Neuropathol Appl Neurobiol

Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.

Authors - Yvonne Davidson
View Article
humanWB,IF

Hum Mol Genet

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Authors - Janis Bennion Callister
View Article
human

Stem Cells

C9orf72 Hexanucleotide Expansions Are Associated with Altered Endoplasmic Reticulum Calcium Homeostasis and Stress Granule Formation in Induced Pluripotent Stem Cell-Derived Neurons from Patients with Amyotrophic Lateral Sclerosis and Frontotemporal Dementia.

Authors - Ruxandra Dafinca
View Article
humanWB

J Biol Chem

eIF5 stimulates the CUG initiation of RAN translation of poly-GA dipeptide repeat protein (DPR) in C9orf72 FTLD/ALS

Authors - Shiho Gotoh
View Article