Flag tag Antibody 5 Publications

Mouse Monoclonal| Catalog number: 60002-1-Ig

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Freight/Packing

Size: 09µ/gram

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Species specificity:
Recombinant protein

Positive WB detected in:
Recombinant Protein

Positive IP detected in:
Recombinant protein protein

Recommended dilution:
WB : 1:2000-1:20000
IP : 1:1000-1:10000

Product Information


Source:
Mouse

Purification method:
N/A

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.

Immunogen Information


Immunogen:
Peptide

Full name:
Flag tag

Calculated molecular weight:

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
DYKDDDDK, Flag, Flag M2, Flag Tag, FlagM2
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects FLAG-tagged proteins.


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