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  • Featured Product
  • KD/KO Validated

FZR1/Cdh1 Polyclonal antibody

FZR1/Cdh1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 16368-1-AP

Synonyms

FZR1, Fizzy related protein homolog, Cdh1/Hct1 homolog, CDH1, CDC20-like protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    WB analysis using 16368-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1/Cdh1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    WB analysis of C6 using 16368-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    WB analysis of NIH/3T3 using 16368-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    WB analysis of HepG2 using 16368-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    WB analysis of mouse liver using 16368-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    WB analysis of mouse heart using 16368-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16368-1-AP (FZR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    IP experiment of mouse heart using 16368-1-AP

    IP result of anti-FZR1/Cdh1 (IP:16368-1-AP, 4ug; Detection:16368-1-AP 1:500) with mouse heart tissue lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    IHC staining of human liver using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    IHC staining of human liver using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    IHC staining of human breast cancer using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    IHC staining of human breast cancer using 16368-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16368-1-AP (FZR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    IF Staining of HepG2 using 16368-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16368-1-AP (FZR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    IF Staining of HeLa using 16368-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16368-1-AP (FZR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using FZR1/Cdh1 Polyclonal antibody (16368-1-AP)

    FC experiment of HeLa using 16368-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human FZR1/Cdh1 (16368-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHMy2.CIR cells, C6 cells, HepG2 cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, HeLa cells, Jurkat cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16368-1-AP targets FZR1/Cdh1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FZR1/Cdh1 fusion protein Ag9201
Full Name fizzy/cell division cycle 20 related 1 (Drosophila)
Calculated Molecular Weight 493 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC013413
Gene Symbol FZR1
Gene ID (NCBI) 51343
RRIDAB_2107380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule. It has 55 kDa, 54 kDa and 45 kDa isoforms.

Protocols

Product Specific Protocols
WB protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IHC protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IF protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
IP protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
FC protocol for FZR1/Cdh1 antibody 16368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
View Article
mouseWB

Sci Adv

The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes.

Authors - Changyin Zhou
  • KD Validated
View Article
humanWB

Acta Pharmacol Sin

Bergenin, a PPARγ agonist, inhibits Th17 differentiation and subsequent neutrophilic asthma by preventing GLS1-dependent glutaminolysis.

Authors - Ling Yang
View Article
mouseWB

Mol Cell Biol

ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction.

Authors - Marie-Claude Perry
View Article
IP,WB

iScience

Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.

Authors - Arielle N Valdez-Sinon
  • KD Validated
View Article
mouseFC

Cell Death Dis

A Cdh1-FoxM1-Apc axis controls muscle development and regeneration.

Authors - Zhe Chen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Chun (Verified Customer) (09-07-2020)

This antibody appears to be good for immunoblotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HaCaT and HEK293 cells