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FXYD3 Polyclonal antibody

FXYD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15853-1-AP

Synonyms

FXYD3, Mammary tumor 8 kDa protein, MAT 8, MAT8, Phospholemman like, PLML

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using FXYD3 Polyclonal antibody (15853-1-AP)

    WB analysis of COLO 320 using 15853-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using FXYD3 Polyclonal antibody (15853-1-AP)

    WB analysis of A375 using 15853-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using FXYD3 Polyclonal antibody (15853-1-AP)

    WB analysis of SGC-7901 using 15853-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using FXYD3 Polyclonal antibody (15853-1-AP)

    IP experiment of COLO 320 using 15853-1-AP

    IP result of anti-FXYD3 (IP:15853-1-AP, 4ug; Detection:15853-1-AP 1:500) with COLO 320 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FXYD3 Polyclonal antibody (15853-1-AP)

    IHC staining of human breast cancer using 15853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FXYD3 Polyclonal antibody (15853-1-AP)

    IHC staining of human breast cancer using 15853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells, A375 cells, SGC-7901 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15853-1-AP targets FXYD3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXYD3 fusion protein Ag8672
Full Name FXYD domain containing ion transport regulator 3
Calculated Molecular Weight 87 aa, 9 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC005238
Gene Symbol FXYD3
Gene ID (NCBI) 5349
RRIDAB_2108602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FXYD3 antibody 15853-1-APDownload protocol
IHC protocol for FXYD3 antibody 15853-1-APDownload protocol
IP protocol for FXYD3 antibody 15853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols