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  • Featured Product
  • KD/KO Validated

FXR2 Polyclonal antibody

FXR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 12552-1-AP

Synonyms

RNA-binding protein FXR2, FXR2P, FMR1L2, FMR1 autosomal homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FXR2 Polyclonal antibody (12552-1-AP)

    WB analysis of HeLa using 12552-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FXR2 Polyclonal antibody (12552-1-AP)

    WB analysis using 12552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FXR2 Polyclonal antibody (12552-1-AP)

    WB analysis of human brain using 12552-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FXR2 Polyclonal antibody (12552-1-AP)

    IP experiment of HeLa using 12552-1-AP

    IP result of anti-FXR2 (IP:12552-1-AP, 4ug; Detection:12552-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FXR2 Polyclonal antibody (12552-1-AP)

    IHC staining of mouse brain using 12552-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FXR2 Polyclonal antibody (12552-1-AP)

    IHC staining of mouse brain using 12552-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FXR2 Polyclonal antibody (12552-1-AP)

    IF Staining of HepG2 using 12552-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FXR2 Polyclonal antibody (12552-1-AP)

    IF Staining of HepG2 using 12552-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, human brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12552-1-AP targets FXR2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXR2 fusion protein Ag3402
Full Name fragile X mental retardation, autosomal homolog 2
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC020090
Gene Symbol FXR2
Gene ID (NCBI) 9513
RRIDAB_2110720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXR2 (FMR1 autosomal homolog 2), also known as: FXR2P; Fxr2h, is belong to the Fragile X gene family. FXR2 is a mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for adult hippocampal neurogenesis. Also, FXR2 acts as a regulator of adult hippocampal neurogenesis by regulating translation and/or stability of NOG mRNA, thereby preventing NOG protein expression in the dentate gyrus(Uniprot).

Protocols

Product Specific Protocols
WB protocol for FXR2 antibody 12552-1-APDownload protocol
IHC protocol for FXR2 antibody 12552-1-APDownload protocol
IF protocol for FXR2 antibody 12552-1-APDownload protocol
IP protocol for FXR2 antibody 12552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope

Authors - Yongrong Liao
View Article
humanRIP

Elife

The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

Authors - Belinda Baquero-Perez
View Article
humanWB

Proc Natl Acad Sci U S A

ARL6IP1 mediates small-molecule-induced alleviation of Alzheimer pathology through FXR1-dependent BACE1 translation initiation

Authors - Gui-Feng Zhou
  • KD Validated
View Article