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  • Featured Product
  • KD/KO Validated

FUT9 Monoclonal antibody

FUT9 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

5D4G6

Cat no : 60230-1-Ig

Synonyms

Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of pig brain using 60230-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of rat brain using 60230-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of mouse brain using 60230-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    WB analysis of fetal human brain using 60230-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human cervical cancer using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human cervical cancer using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human breast cancer using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human breast cancer using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IHC staining of human stomach using 60230-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using FUT9 Monoclonal antibody (60230-1-Ig)

    IF Staining of human breast cancer using 60230-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FUT9 antibody (60230-1-Ig, Clone: 5D4G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60230-1-Ig targets FUT9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FUT9 fusion protein Ag8298
Full Name fucosyltransferase 9 (alpha (1,3) fucosyltransferase)
Calculated Molecular Weight 359 aa, 42 kDa
Observed Molecular Weight 44-46 kDa
GenBank Accession NumberBC036101
Gene Symbol FUT9
Gene ID (NCBI) 10690
RRIDAB_11183046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.

Protocols

Product Specific Protocols
WB protocol for FUT9 antibody 60230-1-IgDownload protocol
IHC protocol for FUT9 antibody 60230-1-IgDownload protocol
IF protocol for FUT9 antibody 60230-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cancers (Basel)

FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.

Authors - Athanasios Blanas
  • KO Validated
View Article
humanWB,IHC

Acta Biochim Biophys Sin (Shanghai)

ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9

Authors - Aiping Xu
  • KD Validated
View Article