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FUT10 Polyclonal antibody

FUT10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18660-1-AP

Synonyms

Fuc TX, Fucosyltransferase 10, Fucosyltransferase X, FucT X, FUT10

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FUT10 Polyclonal antibody (18660-1-AP)

    WB analysis of HeLa using 18660-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18660-1-AP (FUT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using FUT10 Polyclonal antibody (18660-1-AP)

    IHC staining of human testis using 18660-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using FUT10 Polyclonal antibody (18660-1-AP)

    IHC staining of human testis using 18660-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18660-1-AP is FUT10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUT10 fusion protein Ag13415
Full Name fucosyltransferase 10 (alpha (1,3) fucosyltransferase)
Calculated Molecular Weight 521 aa, 61 kDa
Observed Molecular Weight 41-48 kDa
GenBank Accession NumberBC063462
Gene Symbol FUT10
Gene ID (NCBI) 84750
RRIDAB_10641997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FUT10 antibody 18660-1-APDownload protocol
IHC protocol for FUT10 antibody 18660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Elife

The role of the NMD factor UPF3B in olfactory sensory neurons.

Authors - Kun Tan
View Article
humanWB

Arthritis Res Ther

Elevation of α-1,3 fucosylation promotes the binding ability of TNFR1 to TNF-α and contributes to osteoarthritic cartilage destruction and apoptosis.

Authors - Hanjie Yu
View Article