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FUK Polyclonal antibody

FUK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13541-1-AP

Synonyms

1110046B12Rik, fucokinase, FUK, L fucose kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FUK Polyclonal antibody (13541-1-AP)

    WB analysis using 13541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FUK Polyclonal antibody (13541-1-AP)

    WB analysis using 13541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using FUK Polyclonal antibody (13541-1-AP)

    WB analysis of mouse thymus using 13541-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FUK Polyclonal antibody (13541-1-AP)

    IHC staining of mouse kidney using 13541-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13541-1-AP (FUK Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inMCF-7 cells, BxPC-3 cells, mouse thymus tissue, mouse kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13541-1-AP targets FUK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUK fusion protein Ag4481
Full Name fucokinase
Calculated Molecular Weight 1084 aa, 118 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC032542
Gene Symbol FUK
Gene ID (NCBI) 197258
RRIDAB_2104961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUK, also named FCSK, belongs to the GHMP kinase family. FUK encodes fucokinase, the only enzyme capable of converting L-fucose to fucose-1-phosphate, which will ultimately be used for synthesizing GDP-fucose, the donor substrate for all fucosyltransferases (PMID: 30503518).

Protocols

Product Specific Protocols
WB protocol for FUK antibody 13541-1-APDownload protocol
IHC protocol for FUK antibody 13541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols