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FTSJ2 Polyclonal antibody

FTSJ2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22556-1-AP

Synonyms

FJH1, FtsJ homolog 2 (E. coli), FTSJ2, Protein ftsJ homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    WB analysis of human skeletal muscle using 22556-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22556-1-AP (FTSJ2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    WB analysis of human placenta using 22556-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22556-1-AP (FTSJ2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    WB analysis of human placenta using 22556-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22556-1-AP (FTSJ2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using FTSJ2 Polyclonal antibody (22556-1-AP)

    IP experiment of mouse skeletal muscle using 22556-1-AP

    IP result of anti-FTSJ2 (IP:22556-1-AP, 3ug; Detection:22556-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human placenta tissue
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22556-1-AP is FTSJ2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTSJ2 fusion protein Ag18410
Full Name FtsJ homolog 2 (E. coli)
Calculated Molecular Weight 246 aa, 27 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC114514
Gene Symbol FTSJ2
Gene ID (NCBI) 29960
RRIDAB_2879122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Putative ribosomal RNA methyltransferase 2 (FTSJ2), also named as FJH1 or Protein FTSJ homolog 2 is a 246 amino acid protein, which belongs to the methyl-transferase superfamily. FTSJ2 localizes in the nucleus and is widely expressed in in muscle, placenta, and heart. FTSJ2 functions as a nucleolar RNA methyl-transferase involved in eukaryotic RNA processing and modification.

Protocols

Product Specific Protocols
WB protocol for FTSJ2 antibody 22556-1-APDownload protocol
IP protocol for FTSJ2 antibody 22556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols