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FSHR Polyclonal antibody
FSHR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 22665-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 46 publications below |
| IHC | See 15 publications below |
| IF | See 49 publications below |
| FC | See 3 publications below |
Product Information
22665-1-AP targets FSHR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, bovine, sheep, goat, geese |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FSHR fusion protein Ag18158 |
| Full Name | follicle stimulating hormone receptor |
| Calculated Molecular Weight | 695 aa, 78 kDa |
| Observed Molecular Weight | 71-78 kDa |
| GenBank Accession Number | BC118548 |
| Gene Symbol | FSHR |
| Gene ID (NCBI) | 2492 |
| RRID | AB_2631204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FSHR antibody 22665-1-AP | Download protocol |
| IHC protocol for FSHR antibody 22665-1-AP | Download protocol |
| IF protocol for FSHR antibody 22665-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol. | ||
J Hazard Mater GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline | ||
Autophagy Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1 | ||
Redox Biol Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. | ||
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells | ||
Free Radic Biol Med Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Nolan (Verified Customer) (04-11-2022) | Antibody works better if you stain at room temperature for 2 hours instead of 4C overnight.
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