mouse brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10506-2-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10506-2-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
PC-3 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10506-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10506-2-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10506-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10506-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 10506-2-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10506-2-AP
IP result of anti-NCS1 (IP:10506-2-AP, 4ug; Detection:10506-2-AP 1:1000) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of SH-SY5Y using 10506-2-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NCS1 (10506-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NCS1 (10506-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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mouse brain tissue, A431 cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, PC-3 cells, rat brain tissue, SH-SY5Y cells
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human cerebellum tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse cerebellum tissue
Positive FC (Intra) detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10506-2-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10506-2-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10506-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10506-2-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10506-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10506-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 10506-2-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human cerebellum using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human cerebellum using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human brain using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).
IP Figures
IP experiment of mouse brain using 10506-2-AP
IP result of anti-NCS1 (IP:10506-2-AP, 4ug; Detection:10506-2-AP 1:1000) with mouse brain tissue lysate 4000ug.
IF-P Figures
IF Staining of mouse cerebellum using 10506-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 10506-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 10506-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC (INTRA) Figures
FC experiment of SH-SY5Y using 10506-2-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NCS1 (10506-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
NCS1 Polyclonal antibody
Catalog Number
10506-2-AP
Citations
3
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500 FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF-P, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
