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FNDC3A Polyclonal antibody

FNDC3A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25240-1-AP

Synonyms

FNDC3, FNDC3A, HUGO, KIAA0970

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FNDC3A Polyclonal antibody (25240-1-AP)

    WB analysis of HEK-293 using 25240-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25240-1-AP (FNDC3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using FNDC3A Polyclonal antibody (25240-1-AP)

    WB analysis of mouse placenta using 25240-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 25240-1-AP (FNDC3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using FNDC3A Polyclonal antibody (25240-1-AP)

    IF Staining of U-251 using 25240-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using FNDC3A antibody (25240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, mouse placenta tissue
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25240-1-AP targets FNDC3A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FNDC3A fusion protein Ag18736
Full Name fibronectin type III domain containing 3A
Calculated Molecular Weight 1198 aa, 132 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC132812
Gene Symbol FNDC3A
Gene ID (NCBI) 22862
RRIDAB_2879982
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FNDC3A antibody 25240-1-APDownload protocol
IF protocol for FNDC3A antibody 25240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols