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Kindlin 3 Polyclonal antibody

Kindlin 3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18131-1-AP

Synonyms

FERMT3, MIG2B, MIG2 like protein, MIG 2, Kindlin-3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Jurkat using 18131-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis using 18131-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis using 18131-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Jurkat using 18131-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    WB analysis of Raji using 18131-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Kindlin 3 Polyclonal antibody (18131-1-AP)

    IHC staining of human lymphoma using 18131-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Kindlin 3 Polyclonal antibody (18131-1-AP)

    IHC staining of human lymphoma using 18131-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Kindlin 3 Polyclonal antibody (18131-1-AP)

    IF Staining of HeLa using 18131-1-AP

    Immunofluorescent analysis of HeLa cells using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJ774A.1 cells, Jurkat cells, Raji cells, THP-1 cells, mouse kidney tissue, rat kidney tissue, K-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

18131-1-AP targets Kindlin 3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kindlin 3 fusion protein Ag12713
Full Name fermitin family homolog 3 (Drosophila)
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 72-76 kDa
GenBank Accession NumberBC004347
Gene Symbol Kindlin 3/FERMT3
Gene ID (NCBI) 83706
RRIDAB_2103056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.

Protocols

Product Specific Protocols
WB protocol for Kindlin 3 antibody 18131-1-APDownload protocol
IHC protocol for Kindlin 3 antibody 18131-1-APDownload protocol
IF protocol for Kindlin 3 antibody 18131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

PLoS Biol

Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization

Authors - Brooke R D'Arcy
View Article