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COX20 Polyclonal antibody

COX20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25752-1-AP

Synonyms

FAM36A, FAM 36A, Cytochrome c oxidase assembly protein COX20, mitochondrial

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using COX20 Polyclonal antibody (25752-1-AP)

    WB analysis using 25752-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25752-1-AP (COX20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COX20 Polyclonal antibody (25752-1-AP)

    IHC staining of human prostate cancer using 25752-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COX20 Polyclonal antibody (25752-1-AP)

    IHC staining of human prostate cancer using 25752-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using COX20 Polyclonal antibody (25752-1-AP)

    IHC staining of human endometrial cancer using 25752-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using COX20 Polyclonal antibody (25752-1-AP)

    IHC staining of human endometrial cancer using 25752-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25752-1-AP (COX20 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COX20 Polyclonal antibody (25752-1-AP)

    IF Staining of HeLa using 25752-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using COX20 antibody (25752-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using COX20 Polyclonal antibody (25752-1-AP)

    IF Staining of PC-3 using 25752-1-AP

    Immunofluorescent analysis of PC-3 cells using 25752-1-AP (COX20 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25752-1-AP targets COX20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX20 fusion protein Ag22520
Full Name family with sequence similarity 36, member A
Calculated Molecular Weight 118 aa, 13 kDa
Observed Molecular Weight ~16 kDa
GenBank Accession NumberBC018519
Gene Symbol COX20
Gene ID (NCBI) 116228
RRIDAB_2880224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX20, also name as FAM36A, belongs to the COX20 family. Mutations in COX20 are a novel cause of recessively inherited.

Protocols

Product Specific Protocols
WB protocol for COX20 antibody 25752-1-APDownload protocol
IHC protocol for COX20 antibody 25752-1-APDownload protocol
IF protocol for COX20 antibody 25752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell

Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.

Authors - Sridevi Challa
View Article
mouseWB,IF

Sci Data

Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.

Authors - Shin-Haw Lee
View Article
humanWB,IHC

Front Endocrinol (Lausanne)

Novel prognostic features and personalized treatment strategies for mitochondria-related genes in glioma patients

Authors - Ji Wu
View Article