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EXT2 Polyclonal antibody

EXT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11348-1-AP

Synonyms

Multiple exostoses protein 2, Heparan sulfate co-polymerase subunit EXT1, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, EXT-2, Exostosin 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EXT2 Polyclonal antibody (11348-1-AP)

    WB analysis using 11348-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using EXT2 Polyclonal antibody (11348-1-AP)

    WB analysis of NIH/3T3 using 11348-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EXT2 Polyclonal antibody (11348-1-AP)

    IHC staining of human colon cancer using 11348-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11348-1-AP (EXT2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using EXT2 Polyclonal antibody (11348-1-AP)

    IF Staining of Jurkat using 11348-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, U-937 cells, C6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11348-1-AP targets EXT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXT2 fusion protein Ag1748
Full Name exostoses (multiple) 2
Calculated Molecular Weight 718 aa, 82 kDa
Observed Molecular Weight 70-82 kDa
GenBank Accession NumberBC010058
Gene Symbol EXT2
Gene ID (NCBI) 2132
RRIDAB_2293839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Exostosin-2 (EXT2) is type II transmembrane glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone(PMID: 22660413, 36402845, 36593275).

Protocols

Product Specific Protocols
WB protocol for EXT2 antibody 11348-1-APDownload protocol
IHC protocol for EXT2 antibody 11348-1-APDownload protocol
IF protocol for EXT2 antibody 11348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome

Authors - Valeria De Pasquale
View Article