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EXOSC7 Polyclonal antibody

EXOSC7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25292-1-AP

Synonyms

Ribosomal RNA-processing protein 42, KIAA0116, hRrp42p, Exosome component 7, Exosome complex component RRP42

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of MCF-7 using 25292-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of K-562 using 25292-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of HepG2 using 25292-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of A431 using 25292-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of COLO 320 using 25292-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of HEK-293 using 25292-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of Jurkat using 25292-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    WB analysis of HeLa using 25292-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EXOSC7 Polyclonal antibody (25292-1-AP)

    IHC staining of human kidney using 25292-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using EXOSC7 Polyclonal antibody (25292-1-AP)

    IHC staining of human colon using 25292-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EXOSC7 Polyclonal antibody (25292-1-AP)

    IHC staining of human kidney using 25292-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EXOSC7 Polyclonal antibody (25292-1-AP)

    IHC staining of human kidney using 25292-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using EXOSC7 Polyclonal antibody (25292-1-AP)

    IHC staining of human colon using 25292-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EXOSC7 Polyclonal antibody (25292-1-AP)

    IF Staining of MCF-7 using 25292-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 25292-1-AP (EXOSC7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, A431 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25292-1-AP targets EXOSC7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC7 fusion protein Ag18212
Full Name exosome component 7
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012831
Gene Symbol EXOSC7
Gene ID (NCBI) 23016
RRIDAB_2880011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA

Protocols

Product Specific Protocols
WB protocol for EXOSC7 antibody 25292-1-APDownload protocol
IHC protocol for EXOSC7 antibody 25292-1-APDownload protocol
IF protocol for EXOSC7 antibody 25292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article
humanWB,IF,IP

Cell Rep

ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells

Authors - M Carmen Lafita-Navarro
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Tsimafei (Verified Customer) (12-04-2022)

40µg of total protein were resolved on the gel. Incubation was done ON at 4C

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mESCs
EXOSC7 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:25292-1-AP)