ERO1L AntibodyRabbit Polyclonal
|Tested applications||ELISA, IHC, WB, FC, IP|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Positive WB detected in||mouse ovary tissue, A431 cells|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human pancreas cancer tissue|
|Positive FC detected in||HeLa cells|
|Recommended dilution||WB: 1:200-1:1000|
IP = Immunoprecipitation
- mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12007-1-AP(ERO1L antibody) at dilution of 1:100
- Immunohistochemical of paraffin-embedded human pancreas cancer using 12007-1-AP(ERO1L antibody) at dilution of 1:50 (under 10x lens)
- IP Result of anti-ERO1L (IP:12007-1-AP, 4ug; Detection:12007-1-AP 1:300) with L02 cells lysate 1400ug.
- 1X10^6 HeLa cells were stained with 0.2ug ERO1L antibody (12007-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||ERO1L fusion protein ag2620||Full name||ERO1-like (S. cerevisiae)|
|BC008674||Gene ID (NCBI)||30001|
|Gene symbol||ERO1L||Synonyms||ERO1 alpha, ERO1 L, ERO1 L alpha, ERO1 like (S. cerevisiae), ERO1 like protein alpha, ERO1L, Oxidoreductin 1 L alpha|
ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. This antibody has no cross reaction to ERO1L.