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ERMAP Polyclonal antibody

ERMAP Polyclonal Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21375-1-AP

Synonyms

ERMAP, hERMAP, PRO2801, Radin blood group antigen, RD, SC, Scianna blood group antigen

Validation Data Gallery

Filter:
  • Immunoprecipitation (IP) experiment of MCF-7 cells using ERMAP Polyclonal antibody (21375-1-AP)

    IP experiment of MCF-7 using 21375-1-AP

    IP result of anti-ERMAP (IP:21375-1-AP, 4ug; Detection:21375-1-AP 1:300) with MCF-7 cells lysate 1600ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ERMAP Polyclonal antibody (21375-1-AP)

    IF Staining of MCF-7 using 21375-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 21375-1-AP (ERMAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IP detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21375-1-AP is ERMAP Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERMAP fusion protein Ag15899
Full Name erythroblast membrane-associated protein (Scianna blood group)
Calculated Molecular Weight 475 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC099703
Gene Symbol ERMAP
Gene ID (NCBI) 114625
RRIDAB_2878849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ERMAP antibody 21375-1-APDownload protocol
IP protocol for ERMAP antibody 21375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Immunol

The ligation between ERMAP, galectin-9 and dectin-2 promotes Kupffer cell phagocytosis and antitumor immunity

Authors - Jie Li
View Article