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ERK1/2 Polyclonal antibody
ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
357
Cat no : 11257-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HeLa cells, HEK-293 cells, PC-12 cells, C6 cells, mouse brain tissue, mouse lung tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells, MCF-7 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 349 publications below |
| IHC | See 6 publications below |
| IF | See 11 publications below |
| CoIP | See 1 publications below |
Product Information
11257-1-AP targets ERK1/2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, chicken, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERK1/2 fusion protein Ag1759 |
| Full Name | mitogen-activated protein kinase 3 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 38-44 kDa |
| GenBank Accession Number | BC013992 |
| Gene Symbol | ERK1 |
| Gene ID (NCBI) | 5595 |
| RRID | AB_2139822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
| IHC protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
| IF protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer | ||
Redox Biol Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species | ||
Cancer Res Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells | ||
ACS Appl Mater Interfaces Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels. | ||
J Nanobiotechnology Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis | ||
J Nanobiotechnology ACYP2 functions as an innovative nano-therapeutic target to impede the progression of hepatocellular carcinoma by inhibiting the activity of TERT and the KCNN4/ERK pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Charlotte (Verified Customer) (08-15-2024) | The company suggests us this ERK ab as the other one we bought from them was not working. I overlaid ERK with pERK and it matches nicely. Satisfied of this antibody. Done in milk.
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FH XB (Verified Customer) (10-29-2020) | A reliable ab to detect total ERK1/2 by Western blotting.
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