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  • Featured Product
  • KD/KO Validated

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11257-1-AP

Synonyms

ERK1, ERK, ERK2, ERT2, ERK-1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ERK1/2 Polyclonal antibody (11257-1-AP)

    WB analysis using 11257-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ERK1/2 Polyclonal antibody (11257-1-AP)

    WB analysis of HEK-293 using 11257-1-AP

    WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.

  • Western Blot (WB) analysis of HeLa cells using ERK1/2 Polyclonal antibody (11257-1-AP)

    WB analysis of HeLa using 11257-1-AP

    WB result of ERK1/2 antibody (11257-1-AP, 1:2000) with si-Control and si-ERK1/2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ERK1/2 Polyclonal antibody (11257-1-AP)

    IHC staining of human colon cancer using 11257-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ERK1/2 Polyclonal antibody (11257-1-AP)

    IHC staining of human colon cancer using 11257-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ERK1/2 Polyclonal antibody (11257-1-AP)

    IHC staining of human breast cancer using 11257-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ERK1/2 Polyclonal antibody (11257-1-AP)

    IHC staining of human breast cancer using 11257-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HeLa cells using ERK1/2 Polyclonal antibody (11257-1-AP)

    FC experiment of HeLa using 11257-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, HEK-293 cells, PC-12 cells, C6 cells, mouse brain tissue, mouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11257-1-AP targets ERK1/2 in WB, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, zebrafish, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERK1/2 fusion protein Ag1759
Full Name mitogen-activated protein kinase 3
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38-44 kDa
GenBank Accession NumberBC013992
Gene Symbol ERK1
Gene ID (NCBI) 5595
RRIDAB_2139822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
WB protocol for ERK1/2 antibody 11257-1-APDownload protocol
IHC protocol for ERK1/2 antibody 11257-1-APDownload protocol
FC protocol for ERK1/2 antibody 11257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer

Authors - Yurou Chen
View Article
humanWB

Redox Biol

Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species

Authors - He Zhao
View Article
WB

Cancer Res

Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells

Authors - Li Xia
View Article
mouseWB

EMBO Mol Med

Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice

Authors - Huiting Wang
View Article
mouseWB

ACS Appl Mater Interfaces

Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.

Authors - Chaoqun Li
View Article
mouseWB

J Nanobiotechnology

Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis

Authors - Ni Yang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Charlotte (Verified Customer) (08-15-2024)

The company suggests us this ERK ab as the other one we bought from them was not working. I overlaid ERK with pERK and it matches nicely. Satisfied of this antibody. Done in milk.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: HT29
ERK1/2 Antibody Western Blot validation (1/5000 dilution) in HT29 (Cat no:11257-1-AP)
FH

XB (Verified Customer) (10-29-2020)

A reliable ab to detect total ERK1/2 by Western blotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain
ERK1/2 Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:11257-1-AP)