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ERH Polyclonal antibody

ERH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15974-1-AP

Synonyms

DROER, ERH

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ERH Polyclonal antibody (15974-1-AP)

    WB analysis using 15974-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15974-1-AP (ERH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ERH Polyclonal antibody (15974-1-AP)

    WB analysis using 15974-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15974-1-AP (ERH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ERH Polyclonal antibody (15974-1-AP)

    WB analysis of mouse testis using 15974-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15974-1-AP (ERH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ERH Polyclonal antibody (15974-1-AP)

    IHC staining of mouse testis using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15974-1-AP (ERH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using ERH Polyclonal antibody (15974-1-AP)

    IHC staining of human brain using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15974-1-AP (ERH antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ERH Polyclonal antibody (15974-1-AP)

    IHC staining of human brain using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15974-1-AP (ERH antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ERH Polyclonal antibody (15974-1-AP)

    IHC staining of mouse testis using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15974-1-AP (ERH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15974-1-AP targets ERH in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERH fusion protein Ag8761
Full Name enhancer of rudimentary homolog (Drosophila)
Calculated Molecular Weight 104 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC014301
Gene Symbol ERH
Gene ID (NCBI) 2079
RRIDAB_2098556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ERH antibody 15974-1-APDownload protocol
IHC protocol for ERH antibody 15974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

J Cell Sci

Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.

Authors - Valentina Iadevaia
View Article