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ERGIC3 Polyclonal antibody

ERGIC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16029-1-AP

Synonyms

C20orf47, CGI 54, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERGIC3 Polyclonal antibody (16029-1-AP)

    WB analysis using 16029-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16029-1-AP (ERGIC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ERGIC3 Polyclonal antibody (16029-1-AP)

    IHC staining of mouse brain using 16029-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ERGIC3 Polyclonal antibody (16029-1-AP)

    IHC staining of mouse brain using 16029-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ERGIC3 Polyclonal antibody (16029-1-AP)

    IF Staining of HepG2 using 16029-1-AP

    Immunofluorescent analysis of HepG2 cells using 16029-1-AP (ERGIC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16029-1-AP targets ERGIC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERGIC3 fusion protein Ag8893
Full Name ERGIC and golgi 3
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC009765
Gene Symbol ERGIC3
Gene ID (NCBI) 51614
RRIDAB_2098446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ERGIC3 protein was also over-expressed in lung cancer tissues and cultured cells, and expression of ERGIC3 was correlated with the differentiated degree and histological type of lung cancer (PMID: 23374247). The up-regulation of ERGIC3 could promote cellular migration and proliferation in vitro. ERGIC3 may play an active role in the development and progression of lung cancer (PMID: 23374247). The MW of this protein is 50 kDa, and this antibody specially recognises the 50 kDa protein.

Protocols

Product Specific Protocols
WB protocol for ERGIC3 antibody 16029-1-APDownload protocol
IHC protocol for ERGIC3 antibody 16029-1-APDownload protocol
IF protocol for ERGIC3 antibody 16029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Identification of TRAMs as Sphingolipid-Binding Proteins Using a Photoactivatable and Clickable Short Chain Ceramide Analog.

Authors - Yaqin Deng
View Article