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  • Featured Product
  • KD/KO Validated

ERGIC1 Polyclonal antibody

ERGIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16108-1-AP

Synonyms

ERGIC 32, ERGIC1, ERGIC32, KIAA1181, NET24

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ERGIC1 Polyclonal antibody (16108-1-AP)

    WB analysis of HepG2 using 16108-1-AP

    WB result of ERGIC1 antibody (16108-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC1 transfected HepG2 cells.

  • Western Blot (WB) analysis of HepG2 cells using ERGIC1 Polyclonal antibody (16108-1-AP)

    WB analysis of HepG2 using 16108-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ERGIC1 Polyclonal antibody (16108-1-AP)

    WB analysis of L02 using 16108-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ERGIC1 Polyclonal antibody (16108-1-AP)

    WB analysis of HepG2 using 16108-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ERGIC1 Polyclonal antibody (16108-1-AP)

    WB analysis of mouse liver using 16108-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ERGIC1 Polyclonal antibody (16108-1-AP)

    IP experiment of HepG2 using 16108-1-AP

    IP result of anti-ERGIC1 (IP:16108-1-AP, 3ug; Detection:16108-1-AP 1:500) with HepG2 cells lysate 1720ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERGIC1 Polyclonal antibody (16108-1-AP)

    IHC staining of human liver cancer using 16108-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERGIC1 Polyclonal antibody (16108-1-AP)

    IHC staining of human liver cancer using 16108-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ERGIC1 Polyclonal antibody (16108-1-AP)

    IF Staining of HepG2 using 16108-1-AP

    Immunofluorescent analysis of HepG2 cells using 16108-1-AP (ERGIC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, L02 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16108-1-AP targets ERGIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERGIC1 fusion protein Ag9025
Full Name endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1
Calculated Molecular Weight 290 aa, 33 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC012766
Gene Symbol ERGIC1
Gene ID (NCBI) 57222
RRIDAB_2098435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein.

Protocols

Product Specific Protocols
WB protocol for ERGIC1 antibody 16108-1-APDownload protocol
IHC protocol for ERGIC1 antibody 16108-1-APDownload protocol
IF protocol for ERGIC1 antibody 16108-1-APDownload protocol
IP protocol for ERGIC1 antibody 16108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Sequencing of captive target transcripts identifies the network of regulated genes and functions of primate-specific miR-522.

Authors - Shen Mynn Tan
View Article
humanWB

Cell Rep

Proteomic Profiling of Mammalian COPII and COPI Vesicles.

Authors - Frank Adolf
View Article
humanWB,IF

Nat Commun

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2

Authors - Karsten Nalbach
View Article
humanWB,IF

Cell Rep

E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC

Authors - Feiyu Tang
  • KD Validated
View Article