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  • Featured Product
  • KD/KO Validated

ERG Polyclonal antibody

ERG Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14356-1-AP

Synonyms

Transforming protein ERG, Transcriptional regulator ERG, erg 3

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ERG Polyclonal antibody (14356-1-AP)

    WB analysis of MCF-7 using 14356-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ERG Polyclonal antibody (14356-1-AP)

    WB analysis of MCF-7 using 14356-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ERG Polyclonal antibody (14356-1-AP)

    WB analysis of HEK-293 using 14356-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ERG Polyclonal antibody (14356-1-AP)

    WB analysis of MCF-7 using 14356-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using ERG Polyclonal antibody (14356-1-AP)

    IP experiment of MCF-7 using 14356-1-AP

    IP result of anti-ERG (IP:14356-1-AP, 4ug; Detection:14356-1-AP 1:500) with MCF-7 cells lysate 800ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ERG Polyclonal antibody (14356-1-AP)

    IHC staining of human prostate cancer using 14356-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ERG Polyclonal antibody (14356-1-AP)

    IHC staining of human prostate cancer using 14356-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ERG Polyclonal antibody (14356-1-AP)

    IHC staining of mouse brain using 14356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ERG Polyclonal antibody (14356-1-AP)

    IHC staining of mouse brain using 14356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ERG Polyclonal antibody (14356-1-AP)

    IHC staining of human tonsillitis using 14356-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ERG Polyclonal antibody (14356-1-AP)

    IHC staining of human tonsillitis using 14356-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ERG Polyclonal antibody (14356-1-AP)

    IF Staining of MCF-7 using 14356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14356-1-AP (ERG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using ERG Polyclonal antibody (14356-1-AP)

    FC experiment of MCF-7 using 14356-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human ERG (14356-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, mouse brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14356-1-AP targets ERG in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERG fusion protein Ag5708
Full Name v-ets erythroblastosis virus E26 oncogene homolog (avian)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC040168
Gene Symbol ERG
Gene ID (NCBI) 2078
RRIDAB_2098423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for ERG antibody 14356-1-APDownload protocol
IHC protocol for ERG antibody 14356-1-APDownload protocol
IF protocol for ERG antibody 14356-1-APDownload protocol
IP protocol for ERG antibody 14356-1-APDownload protocol
FC protocol for ERG antibody 14356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

iScience

Resident vascular Sca1+ progenitors differentiate into endothelial cells in vascular remodeling via miR-145-5p/ERG signaling pathway

Authors - Zhangquan Ying
View Article
humanWB

Cell Biosci

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

Authors - Qiong Wang
View Article
humanWB

J Cancer

miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.

Authors - Yongbao Wei
  • KD Validated
View Article
human,mouseWB,IF

Cell Death Discov

HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells.

Authors - Feng Yao
View Article
humanIHC

Exp Ther Med

Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report

Authors - Xiaohua Wang
View Article
humanWB

Nat Commun

DNA framework-engineered chimeras platform enables selectively targeted protein degradation

Authors - Li Zhou
View Article