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- KD/KO Validated
ERCC6L Polyclonal antibody
ERCC6L Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15688-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human heart tissue, Jurkat cells, mouse lung tissue, K-562 cells, U2OS cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
Product Information
15688-1-AP targets ERCC6L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERCC6L fusion protein Ag8223 |
| Full Name | excision repair cross-complementing rodent repair deficiency, complementation group 6-like |
| Calculated Molecular Weight | 419 aa, 46 kDa, 141 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC008808 |
| Gene Symbol | ERCC6L |
| Gene ID (NCBI) | 54821 |
| RRID | AB_2098171 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERCC6L, also named as Tumor antigen BJ-HCC-15 or PICH, is a 1250 amino acid protein, which contains one helicase ATP-binding domain, one helicase C-terminal domain and two TPR repeats. ERCC6L localizes to kinetochores, inner centromeres and belongs to the SNF2/RAD54 helicase family. ERCC6L as a DNA helicase that acts as an essential component of the spindle assembly checkpoint. The calculated molecular weight of ERCC6L is 140 kDa, but the phosphorylated modification of molecular weight of ERCC6L is about 180kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERCC6L antibody 15688-1-AP | Download protocol |
| IHC protocol for ERCC6L antibody 15688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Med Res Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells | ||
J Ethnopharmacol Gypenoside LI arrests the cell cycle of breast cancer in G0/G1 phase by down-regulating E2F1. | ||
Oncol Rep Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma. | ||
BMC Cancer ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-κB signaling pathway.
| ||
Oncol Lett ERCC6L promotes cell growth and invasion in human colorectal cancer.
| ||
Cancer Res PICH activates Cyclin A1 transcription to drive S-phase progression and chemoresistance in gastric cancer |
